CLS 5410 Molecular Diagnostics Laboratory I
This is an introductory lecture-based course with a hands-on component designed to familiarize students with basic molecular and biochemical techniques including but not limited to: nucleic acid extraction and quantification; gel electrophoresis, including both agarose and SDS-polyacrylamide gels; western blotting; and the polymerase chain reaction (PCR), including reverse transcription PCR (RT-PCR) and “real-time†or quantitative PCR (qPCR) - 3 lecture hours.